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p p38  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p p38
    P P38, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 30331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38/p38+MAPK+Antibody/pmc13019078-153-12-23
    Average 99 stars, based on 30331 article reviews
    p p38 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: MerTK inhibition by UNC569 triggers DNA damage and JNK/p38 MAPK cascade-driven apoptosis in pancreatic cancer.
    Article Snippet: .. The following primary antibodies were used: p-Rb (8516), p21 (2947), Snail (3879), cleaved caspase3 (9669), PARP (9542), JNK (9252), p-p38 (4511), γ-H2A.X (9718), p-JNK (4668) and p38 (8690) from Cell Signaling Technology (Danvers, USA); p-MerTK (ab14921) from Abcam (Cambridge, UK); Rb (A3618) and p-CDK6 (AP0326) from ABclonal (Wuhan, China); β-actin (P30002) from Abmart (Shanghai, China); N-cadherin (22018-1-AP) and Ecadherin (20874-1-AP) from Proteintech (Wuhan, China); Cyclin B1 (sc-245) and c-Myc (sc-42) from Santa Cruz Biotechnology (Dallas, USA); CDK6 (VWA00022) and p27 (AHP2662) from BioRad (Hercules, USA). ..

    Article Title: Designed bone-targeting ROS-responsive nanoplatform for precision glycolysis inhibition in postmenopausal osteoporosis
    Article Snippet: Antibodies against osteoclast marker proteins, such as c-Fos, CTSK, integrin β3, and NFATc1, were procured from Santa Cruz (San Jose, United States). .. Moreover, antibodies targeting p-P38, P38, p-JNK, JNK, p-ERK, ERK, p-P65, P65, and IκB-α were sourced from Cell Signaling Technology, Danvers, United States. .. Proteintech (Wuhan, China) provided antibodies for MCT1, MCT2, MMP9, RUNX2, β-actin, HK2, LDHA, and PKM2.

    other:


    Blocking Assay:

    Article Title: Purslane ( Portulaca oleracea L.) Extract Alleviates Atherosclerosis in ApoE −/− Mice
    Article Snippet: Protein samples (15 μg) were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to PVDF membranes (Millipore, Billerica, MA, USA) via wet transfer. .. After blocking with 1% BSA in PBST, membranes were incubated overnight at 4°C with primary antibodies against: ICAM‐1, VCAM‐1, iNOS, PI3K, p‐AMPKα (Thr172), total AMPKα, p‐Akt (Ser473), total Akt, p‐eNOS (Ser1177), total eNOS, p‐JNK (Thr183/Tyr185), total JNK, p‐ERK1/2 (Thr202/Tyr204), total ERK, p‐p38 (Thr180/Tyr182), total p38, and GAPDH (all from Cell Signaling Technology, Danvers, MA, USA). .. Membranes were then incubated with the corresponding HRP‐coupled secondary antibody (Beyotime Biotechnology, Shanghai, China) at room temperature for 2 h. The antibody incubation method was determined based on the recommended method of the reagent supplier and with reference to the study of Luo et al. [ ].

    Article Title: Purslane (Portulaca oleracea L.) Extract Alleviates Atherosclerosis in ApoE -/- Mice.
    Article Snippet: Protein samples (15 μg) were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Millipore, Billerica, MA, USA) via wet transfer. .. After blocking with 1% BSA in PBST, membranes were incubated overnight at 4◦C with primary antibodies against: ICAM-1, VCAM-1, iNOS, PI3K, p-AMPK α (Thr172), total AMPK α, p-Akt (Ser473), total Akt, p-eNOS (Ser1177), total eNOS, pJNK (Thr183/Tyr185), total JNK, p-ERK1/2 (Thr202/Tyr204), total ERK, p-p38 (Thr180/Tyr182), total p38, and GAPDH (all from Cell Signaling Technology, Danvers, MA, USA). .. Membranes were then incubated with the corresponding HRP-coupled secondary antibody (Beyotime Biotechnology, Shanghai, China) at room temperature for 2 h. The antibody incubation method was determined based on the recommended method of the reagent supplier and with reference to the study of Luo et al. [ 14 ].

    Incubation:

    Article Title: Purslane ( Portulaca oleracea L.) Extract Alleviates Atherosclerosis in ApoE −/− Mice
    Article Snippet: Protein samples (15 μg) were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to PVDF membranes (Millipore, Billerica, MA, USA) via wet transfer. .. After blocking with 1% BSA in PBST, membranes were incubated overnight at 4°C with primary antibodies against: ICAM‐1, VCAM‐1, iNOS, PI3K, p‐AMPKα (Thr172), total AMPKα, p‐Akt (Ser473), total Akt, p‐eNOS (Ser1177), total eNOS, p‐JNK (Thr183/Tyr185), total JNK, p‐ERK1/2 (Thr202/Tyr204), total ERK, p‐p38 (Thr180/Tyr182), total p38, and GAPDH (all from Cell Signaling Technology, Danvers, MA, USA). .. Membranes were then incubated with the corresponding HRP‐coupled secondary antibody (Beyotime Biotechnology, Shanghai, China) at room temperature for 2 h. The antibody incubation method was determined based on the recommended method of the reagent supplier and with reference to the study of Luo et al. [ ].

    Article Title: Purslane (Portulaca oleracea L.) Extract Alleviates Atherosclerosis in ApoE -/- Mice.
    Article Snippet: Protein samples (15 μg) were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Millipore, Billerica, MA, USA) via wet transfer. .. After blocking with 1% BSA in PBST, membranes were incubated overnight at 4◦C with primary antibodies against: ICAM-1, VCAM-1, iNOS, PI3K, p-AMPK α (Thr172), total AMPK α, p-Akt (Ser473), total Akt, p-eNOS (Ser1177), total eNOS, pJNK (Thr183/Tyr185), total JNK, p-ERK1/2 (Thr202/Tyr204), total ERK, p-p38 (Thr180/Tyr182), total p38, and GAPDH (all from Cell Signaling Technology, Danvers, MA, USA). .. Membranes were then incubated with the corresponding HRP-coupled secondary antibody (Beyotime Biotechnology, Shanghai, China) at room temperature for 2 h. The antibody incubation method was determined based on the recommended method of the reagent supplier and with reference to the study of Luo et al. [ 14 ].



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    Image Search Results


    The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

    Journal: Poultry Science

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    doi: 10.1016/j.psj.2026.106922

    Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

    Article Snippet: p-p38 MAPK Rabbit Ab , Abmart , TA4001 , 1: 1500.

    Techniques: Expressing

    The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

    Journal: Poultry Science

    Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

    doi: 10.1016/j.psj.2026.106922

    Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

    Article Snippet: p-p38 MAPK Rabbit Ab , Abmart , TA4001 , 1: 1500.

    Techniques: Activity Assay

    Effect of Lut on 42 °C-stressed differential gene and protein expression in cells by transcriptomic analysis and western blot, respectively. (A) A volcano plot illustrating differentially regulated gene expression between HS and HS + Lut (10 μM). Genes upregulated and downregulated are shown in red and blue, respectively. (B) Top 20 of GO enrichment about molecular function. (C) Top 20 of GO enrichment about biological process. (D) Top 20 of KEGG enrichment. The color and size of the bubbles represented the significance of the processes and the number of genes, respectively. (E, F) Effect of Lut (10 μM) on total p-p38, p38, Hsp70, and Hsp90 protein expression. β-actin was used as an internal control for proteins. n = 3. Data are expressed as mean ± SD. * represents the significant difference compared with the control group ( P < 0.05), # represents the significant difference compared with the HS ( P < 0.05).

    Journal: Poultry Science

    Article Title: Targeted intestinal delivery of luteolin microcapsules as a precision nutritional strategy to alleviate heat stress and enhance growth performance in broilers

    doi: 10.1016/j.psj.2026.106976

    Figure Lengend Snippet: Effect of Lut on 42 °C-stressed differential gene and protein expression in cells by transcriptomic analysis and western blot, respectively. (A) A volcano plot illustrating differentially regulated gene expression between HS and HS + Lut (10 μM). Genes upregulated and downregulated are shown in red and blue, respectively. (B) Top 20 of GO enrichment about molecular function. (C) Top 20 of GO enrichment about biological process. (D) Top 20 of KEGG enrichment. The color and size of the bubbles represented the significance of the processes and the number of genes, respectively. (E, F) Effect of Lut (10 μM) on total p-p38, p38, Hsp70, and Hsp90 protein expression. β-actin was used as an internal control for proteins. n = 3. Data are expressed as mean ± SD. * represents the significant difference compared with the control group ( P < 0.05), # represents the significant difference compared with the HS ( P < 0.05).

    Article Snippet: Anti-HSP90 antibody and Anti-p-p38 antibody were obtained from Cohesion Biosciences Co., Ltd. (England).

    Techniques: Expressing, Western Blot, Gene Expression, Control

    Microcapsule inhibits HS-induced protein expression of p-p38/p38, Hsp70, and Hsp90 in the liver. (A) Effect of microcapsule on total p-p38, p38, Hsp70, and Hsp90 protein expression in liver. (B) Analysis of protein abundance. β-actin was used as an internal control for proteins, n = 3. Data are expressed as mean ± SD. * represents the significant difference compared with the HS group ( P < 0.05). ** represents the highly significant difference compared with the HS group ( P < 0.01). No symbol represents no difference compared with the HS group ( P > 0.05).

    Journal: Poultry Science

    Article Title: Targeted intestinal delivery of luteolin microcapsules as a precision nutritional strategy to alleviate heat stress and enhance growth performance in broilers

    doi: 10.1016/j.psj.2026.106976

    Figure Lengend Snippet: Microcapsule inhibits HS-induced protein expression of p-p38/p38, Hsp70, and Hsp90 in the liver. (A) Effect of microcapsule on total p-p38, p38, Hsp70, and Hsp90 protein expression in liver. (B) Analysis of protein abundance. β-actin was used as an internal control for proteins, n = 3. Data are expressed as mean ± SD. * represents the significant difference compared with the HS group ( P < 0.05). ** represents the highly significant difference compared with the HS group ( P < 0.01). No symbol represents no difference compared with the HS group ( P > 0.05).

    Article Snippet: Anti-HSP90 antibody and Anti-p-p38 antibody were obtained from Cohesion Biosciences Co., Ltd. (England).

    Techniques: Expressing, Quantitative Proteomics, Control

    Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The phosphorylation of Smad1/5/9 and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Inflammation-induced osteogenic signaling promotes calcium phosphate crystal formation in kidneys via MAPK, NF-κB, and smad pathways

    doi: 10.3389/fcell.2026.1831072

    Figure Lengend Snippet: Cytokines Activated Their Downstream Pathways in HK-2 Cells. The phosphorylation of ERK, JNK, p38, and NF-κB in HK-2 cells treated with TNF-α (A) or IL-1β (B) was analyzed over a time course ranging from 15 min to 4 h. The phosphorylation of Smad1/5/9 and Smad2/3 in HK-2 cells treated with TGF-β1 (C) or BMP-2 (D) was analyzed over a time course ranging from 15 min to 4 h. GAPDH was served as the loading control.

    Article Snippet: Primary antibodies for immunoblotting such as OPN (A19092), ALP (A0514), OPG (A2100), Runx2 (A2851), p-JNK1/2/3 (AP0631), JNK1/2/3 (A4867), p-NF-κB (AP0944), NF-κB (A19653), p-p38 (AP0057), p38 (A14401), p-ERK (AP0485), ERK (A4782), p-Smad1/5/9 (AP0850), Smad1 (A21734), p-Smad2/3 (AP0548), Smad2/3 (A7536), TNF-α (A11534), IL-1β (A11534), TGF-β1 (A16640), BMP-2 (A0231), β-actin (AC028), α-Tubulin (A6830), and GAPDH (A19056) were from ABclonal (Woburn, MA, United States).

    Techniques: Phospho-proteomics, Control

    Pro-inflammatory Cytokines Upregulated Osteogenesis-related Proteins via the MAPK/NF-κB and Smad Signaling Pathway. HK-2 cells were pretreated with 10 µM ERK inhibitor (U0126), JNK inhibitor (SP600125), p38 inhibitor (SB203580), or NF-κB inhibitor (BAY 11–7,082) for 2 h, and then treated with 20 ng/mL TNF-α (A) or IL-1β (B) for 72 h (C) HK-2 cells were pretreated with 5 µM Smad2/3 inhibitor (SB-525334) for 2 h and then treated with 200 ng/mL TGF-β1 for 72 h (D) HK-2 cells were pretreated with 10 µM Smad1/5/8 inhibitor (LDN-193189) for 2 h and then treated with 100 ng/mL BMP-2 for 72 h. The expression of osteoblast markers (ALP, OPN, and OPG) was examined by Western blotting. The quantitative results showed the fold change of the protein expression compared to the control group. GAPDH served as the loading control. Data are presented as individual data points (scatter plot) with the mean ± SEM; n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the control group; # P < 0.05, ## P < 0.01, ### P < 0.001 versus cytokine group (One-way ANOVA).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Inflammation-induced osteogenic signaling promotes calcium phosphate crystal formation in kidneys via MAPK, NF-κB, and smad pathways

    doi: 10.3389/fcell.2026.1831072

    Figure Lengend Snippet: Pro-inflammatory Cytokines Upregulated Osteogenesis-related Proteins via the MAPK/NF-κB and Smad Signaling Pathway. HK-2 cells were pretreated with 10 µM ERK inhibitor (U0126), JNK inhibitor (SP600125), p38 inhibitor (SB203580), or NF-κB inhibitor (BAY 11–7,082) for 2 h, and then treated with 20 ng/mL TNF-α (A) or IL-1β (B) for 72 h (C) HK-2 cells were pretreated with 5 µM Smad2/3 inhibitor (SB-525334) for 2 h and then treated with 200 ng/mL TGF-β1 for 72 h (D) HK-2 cells were pretreated with 10 µM Smad1/5/8 inhibitor (LDN-193189) for 2 h and then treated with 100 ng/mL BMP-2 for 72 h. The expression of osteoblast markers (ALP, OPN, and OPG) was examined by Western blotting. The quantitative results showed the fold change of the protein expression compared to the control group. GAPDH served as the loading control. Data are presented as individual data points (scatter plot) with the mean ± SEM; n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001 versus the control group; # P < 0.05, ## P < 0.01, ### P < 0.001 versus cytokine group (One-way ANOVA).

    Article Snippet: Primary antibodies for immunoblotting such as OPN (A19092), ALP (A0514), OPG (A2100), Runx2 (A2851), p-JNK1/2/3 (AP0631), JNK1/2/3 (A4867), p-NF-κB (AP0944), NF-κB (A19653), p-p38 (AP0057), p38 (A14401), p-ERK (AP0485), ERK (A4782), p-Smad1/5/9 (AP0850), Smad1 (A21734), p-Smad2/3 (AP0548), Smad2/3 (A7536), TNF-α (A11534), IL-1β (A11534), TGF-β1 (A16640), BMP-2 (A0231), β-actin (AC028), α-Tubulin (A6830), and GAPDH (A19056) were from ABclonal (Woburn, MA, United States).

    Techniques: Expressing, Western Blot, Control

    YHD exerts anti-tumor effects on osteosarcoma (OS) cells through the PI3K/AKT and p38 signaling pathways. (A) Principal component analysis revealed a clear distinction in gene expression profiles between the control and YHD groups. (B) Volcano plot identified 3495 differentially expressed genes in the YHD group. (C) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. (D – G) Gene Set Enrichment Analysis (GSEA) of control and YHD groups. (H, I) Western blot analysis detected the effect of YHD on proteins related to the PI3K/AKT and MAPK pathways in OS cells. (J, K) After the addition of a PI3K activator and a P38 inhibitor, scratch healing assay showed that YHD inhibited the migration of OS cells. (L, M) After the addition of a PI3K activator and a P38 inhibitor, JC-1 staining detected the effect of YHD on the mitochondrial membrane potential in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Journal: Genes & Diseases

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

    doi: 10.1016/j.gendis.2025.101862

    Figure Lengend Snippet: YHD exerts anti-tumor effects on osteosarcoma (OS) cells through the PI3K/AKT and p38 signaling pathways. (A) Principal component analysis revealed a clear distinction in gene expression profiles between the control and YHD groups. (B) Volcano plot identified 3495 differentially expressed genes in the YHD group. (C) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. (D – G) Gene Set Enrichment Analysis (GSEA) of control and YHD groups. (H, I) Western blot analysis detected the effect of YHD on proteins related to the PI3K/AKT and MAPK pathways in OS cells. (J, K) After the addition of a PI3K activator and a P38 inhibitor, scratch healing assay showed that YHD inhibited the migration of OS cells. (L, M) After the addition of a PI3K activator and a P38 inhibitor, JC-1 staining detected the effect of YHD on the mitochondrial membrane potential in OS cells. Data were presented as mean ± standard deviation ( n = 3). ∗ p < 0.05 and ∗∗ p < 0.01 versus the blank group.

    Article Snippet: Cyclin B (#4138), Vimentin (#5741), N-cadherin (#13116), p38 (#9212), and p-p38 (#4511S) antibodies were purchased from Cell Signaling Technology (USA).

    Techniques: Protein-Protein interactions, Gene Expression, Control, Western Blot, Migration, Staining, Membrane, Standard Deviation

    YHD inhibits the growth of OS in vivo . (A) The effect of YHD on tumorigenesis in nude mice. (B) The effect of YHD on tumor volume. (C) The effect of YHD on mouse weight. (D) Hematoxylin-eosin staining of the mouse tumor tissue. (E) Hematoxylin-eosin staining of the mouse lung tissue. (F) PCNA, Bcl-2, Vimentin, p-AKT, and p-P38 of the mouse tumor tissue were detected by immunohistochemistry. (G) Hematoxylin-eosin staining of the mouse heart and liver tissue. Data were presented as mean ± standard deviation ( n = 6).

    Journal: Genes & Diseases

    Article Title: Network pharmacology reveals that Yanghe Decoction inhibits osteosarcoma progression via ROS-induced mitochondrial dysfunction and enhances cisplatin sensitivity

    doi: 10.1016/j.gendis.2025.101862

    Figure Lengend Snippet: YHD inhibits the growth of OS in vivo . (A) The effect of YHD on tumorigenesis in nude mice. (B) The effect of YHD on tumor volume. (C) The effect of YHD on mouse weight. (D) Hematoxylin-eosin staining of the mouse tumor tissue. (E) Hematoxylin-eosin staining of the mouse lung tissue. (F) PCNA, Bcl-2, Vimentin, p-AKT, and p-P38 of the mouse tumor tissue were detected by immunohistochemistry. (G) Hematoxylin-eosin staining of the mouse heart and liver tissue. Data were presented as mean ± standard deviation ( n = 6).

    Article Snippet: Cyclin B (#4138), Vimentin (#5741), N-cadherin (#13116), p38 (#9212), and p-p38 (#4511S) antibodies were purchased from Cell Signaling Technology (USA).

    Techniques: In Vivo, Staining, Immunohistochemistry, Standard Deviation